荧光光谱法研究单壁碳纳米管与牛血红蛋白的相互作用

杨兴; 谭惠心; 章弘扬; 胡坪; 张敏

doi:10.14135/J.cnki.1006-3080.20211018003

荧光光谱法研究单壁碳纳米管与牛血红蛋白的相互作用

doi: 10.14135/J.cnki.1006-3080.20211018003

杨兴^1,,
谭惠心¹,
章弘扬²,
胡坪²,
张敏^1,

1.
华东理工大学药学院, 制药工程与过程化学教育部工程研究中心
2.
华东理工大学化学与分子工程学院, 上海市功能性材料化学重点实验室, 上海200237

基金项目: 国家自然科学基金（81573397）

详细信息

作者简介:
杨兴：杨　兴（1997—），男，江西人，研究生，研究方向为药物分析。E-mail：1500538191@qq.com

中图分类号: O657.3
计量
- 文章访问数: 31
- HTML全文浏览量: 28
- PDF下载量: 5
- 被引次数: 0
出版历程
- 收稿日期: 2021-10-18
- 录用日期: 2022-01-11
- 网络出版日期: 2022-04-12

Fluorescence Spectroscopic Investigation of the Interaction of Single-walled Carbon Nanotubes with Bovine Hemoglobin

1.
Engineering Research Center of Ministry of Education for Pharmaceutical Engineering and Process Chemistry, School of Pharmacy, East China University of Science and Technology
2.
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

摘要

摘要: 选取了SWCNTs及两种分离后的单一手性单壁碳纳米管（(6,5)-SWCNT，(8,3)-SWCNT）分别与牛血红蛋白（BHb）结合，通过荧光光谱法分析SWCNTs与BHb的相互作用。结果表明，SWCNTs对BHb的荧光猝灭是通过动态猝灭与静态猝灭结合的机制，单一手性(6,5)-SWCNT和(8,3)-SWCNT对BHb的荧光猝灭是静态猝灭的机制；BHb与3种SWCNT的结合常数大小顺序如下：SWCNTs＞(6,5)-SWCNT＞(8,3)-SWCNT；范德华力、氢键和疏水作用是相互作用中主要作用力。
- 单壁碳纳米管 /
- 牛血红蛋白 /
- 相互作用 /
- 荧光光谱 /
- 猝灭
Abstract: Single-walled carbon nanotubes (SWCNTs) can combine with proteins in organisms, creating potential biosafety risks. In this paper, SWCNTs and two separated single-chiral single-walled carbon nanotubes ((6,5)-SWCNT, (8,3)-SWCNT) were combined with Bovine hemoglobin (BHb) respectively, and SWCNTs interaction with BHb were analyzed by fluorescence spectroscopy. The results showed that the fluorescence quenching of BHb by SWCNTs was resulted from the combination of dynamic quenching and static quenching. The fluorescence quenching of BHb by single chiral (6,5)-SWCNT and (8,3)-SWCNT was static quenching. The order of the binding constants of BHb and different SWCNTs was as follows: SWCNTs>(6,5)-SWCNT>(8,3)-SWCNT. Van der Waals force, hydrogen bond and hydrophobic interaction were the main forces in the interaction. The result of this article will assist the revealing of the potential biosafety risks of SWCNTs.
- Single-walled carbon nanotubes /
- Bovine hemoglobin /
- Interaction /
- Fluorescence spectrum /
- Quenching

HTML全文

图 1 SWCNTs、(6,5)-SWCNT和(8,3)-SWCNT的紫外-可见-近红外吸收光谱

Figure 1. UV-Vis-NIR absorption spectra of SWCNTs, (6,5)-SWCNT and (8,3)-SWCNT

The inset is digital photograph of (a) SWCNTs dispersion, (b) Separated (6,5)-SWCNT,(c) Separated (8,3)-SWCNT

下载: 全尺寸图片幻灯片

图 2 SWCNTs对BHb荧光光谱的影响

Figure 2. The effect of SWCNTs on the fluorescence spectra of BHb

下载: 全尺寸图片幻灯片

图 3 BHb荧光在四种不同温度和 pH 7.40 下被(a)SWCNTs，(b)(6,5)-SWCNT，(c)(8,3)-SWCNT猝灭的 Stern-Volmer 图

Figure 3. Stern-Volmer plot of BHb fluorescence quenched by original SWCNTs (a), (6,5)-SWCNT (b), (8,3)-SWCNT (c) at four different temperatures and pH 7.40

下载: 全尺寸图片幻灯片

图 4 不同温度下SWCNTs-BHb的 ln ( F₀ / F_I ) 与 [ Q ] 关系图

Figure 4. The relationship between ln (F₀/F_I) and [Q] of SWCNTs-BHb at different temperatures

下载: 全尺寸图片幻灯片

图 5 不同温度下SWCNTs-BHb的 lg ( F₀ − F _I)/ F_I 与 lg [Q]关系图

Figure 5. The relationship between lg (F₀-F_I)/F_I and lg [Q] of SWCNTs-BHb at different temperatures

下载: 全尺寸图片幻灯片

图 6 不同 SWCNT-BHb 系统的lnK_A对1/T的关系图

Figure 6. The relationship between lnK_A and 1/T of different SWCNT-BHb systems

下载: 全尺寸图片幻灯片

表 1 在不同温度下SWCNT-BHb系统的Stern-Volmer猝灭常数

Table 1. Stern-Volmer quenching constants of different SWCNT-BHb systems at different temperatures

	T/K	K_SV/ (10³ L·mol^-1)	K_q/ [10¹² L/(mol·s)]	R²	V/ (10³ L·mol^-1)	K/ (10³ L·mol^-1)	R²
SWCNTs	298	13.05	2.58	0.993	9.82	5.46	0.994
	308	10.39	2.05	0.997	8.13	5.40	0.989
	318	9.80	1.94	0.997	7.62	5.47	0.982
	328	8.60	1.70	0.995	6.78	4.94	0.988
(6,5)- SWCNT	298	5.95	1.18	0.983	/	/	/
	308	5.93	1.17	0.983	/	/	/
	318	5.56	1.10	0.983	/	/	/
	328	5.32	1.05	0.981	/	/	/
(8,3)- SWCNT	298	5.44	1.08	0.964	/	/	/
	308	5.24	1.04	0.993	/	/	/
	318	4.56	0.90	0.992	/	/	/
	328	4.23	0.84	0.991	/	/	/

下载: 导出CSV

表 2 在不同温度下 SWCNT-BHb 系统的结合常数和结合位点数

Table 2. Binding constants and number of binding sites for different SWCNT-BHb systems at different temperatures

	T/K	K_A/(L·mol^-1)	n	R²
SWCNTs	298	1.39×10⁶	1.48	0.986
	308	1.96×10⁵	1.30	0.976
	318	7.71×10⁴	1.20	0.956
	328	2.50×10⁴	1.10	0.961
(6,5)-SWCNT	298	7.93×10²	0.83	0.979
	308	1.71×10³	0.90	0.978
	318	2.49×10³	0.93	0.980
	328	3.65×10³	0.92	0.975
(8,3)-SWCNT	298	1.03×10²	0.67	0.978
	308	5.94×10²	0.82	0.988
	318	4.81×10³	1.00	0.992
	328	2.49×10⁴	1.14	0.986

下载: 导出CSV

表 3 SWCNT与BHb相互作用的热力学参数

Table 3. Thermodynamic parameters of the interaction between SWCNTs and BHb

	∆H/(kJ·mol^-1)	∆G/(kJ·mol^-1)	∆S/(J·mol^-1·K^-1)	R²
SWCNTs	−105.93	−34.50	−239.69	0.968
	−105.93	−32.10	−239.69	0.968
	−105.93	−29.71	−239.69	0.968
	−105.93	−27.31	−239.69	0.968
(6,5)−SWCNT	40.46	−16.78	192.07	0.960
	40.46	−18.70	192.07	0.960
	40.46	−20.62	192.07	0.960
	40.46	−22.54	192.07	0.960
(8,3)−SWCNT	150.73	−11.31	543.77	0.997
	150.73	−16.75	543.77	0.997
	150.73	−22.18	543.77	0.997
	150.73	−27.62	543.77	0.997

下载: 导出CSV

参考文献(18)

[1]	ODOM T W, HUANG J L, KIM P, et al. Atomic structure and electronic properties of single-walled carbon nanotubes[J]. Nature, 1998, 391(6662): 62-64. doi: 10.1038/34145
[2]	RUOFF R S, DONG Q, LIU W K. Mechanical properties of carbon nanotubes: Theoretical predictions and experimental measurements[J]. Comptes Rendus Physique, 2003, 4(9): 993-1008. doi: 10.1016/j.crhy.2003.08.001
[3]	RUOFF R S. Carbon nanotubes \| \| Mechanical and thermal properties of carbon nanotubes[J], 1996: 143-148.
[4]	BACHILO S M, STRANO M S, KITTRELL C, et al. Structure-assigned optical spectra of single-walled carbon nanotubes[J]. Science, 2002, 298(5602): 2361-2366. doi: 10.1126/science.1078727
[5]	SK A, BS B, TCJ A. Theorical investigation of adsorption mechanism of doxorubicin anticancer drug on the pristine and functionalized single-walled carbon nanotube surface as a drug delivery vehicle: A dft study - sciencedirect[J]. Journal of Molecular Liquids, 2020: 322.
[6]	ZHAO Z, MA R, GUO Z R, et al. Single-walled carbon nanotubes as drug carrier loaded with moroxydine hydrochloride against infectious spleen and kidney necrosis virus in mandarin fish[J]. Aquaculture, 2021(4): 736469.
[7]	ZHANG H, WU B, HU W, et al. Separation and/or selective enrichment of single-walled carbon nanotubes based on their electronic properties[J]. Chemical Society Reviews, 2011, 40(3): 1324-1336. doi: 10.1039/B920457C
[8]	CHEN M, ZHU Y, ZHOU S, et al. Toxicity of carbon nanomaterials to plants, animals and microbes: Recent progress from 2015-present[J]. Chemosphere, 2018, 206: 255-264. doi: 10.1016/j.chemosphere.2018.05.020
[9]	仲文博, 张敏, 章弘扬, 等. (6, 5)单壁碳纳米管对盐酸阿霉素的载药研究[J]. 华东理工大学学报(自然科学版), 2021, 47(1): 41-47.
[10]	WANG Y Q, ZHANG H M, JIAN C. Binding of hydroxylated single-walled carbon nanotubes to two hemoproteins, hemoglobin and myoglobin[J]. Journal of Photochemistry and Photobiology A-Chemistry, 2014, 141: 26-35. doi: 10.1016/j.jphotobiol.2014.08.020
[11]	FAGAN J A, KHRIPIN C Y, BATISTA C, et al. Isolation of specific small‐diameter single‐wall carbon nanotube species via aqueous two‐phase extraction[J]. Journal of Advanced Materials, 2014, 26(18): 2800-2804. doi: 10.1002/adma.201304873
[12]	WEISMAN R B, BACHILO S M. Dependence of optical transition energies on structure for single-walled carbon nanotubes in aqueous suspension: An empirical kataura plot[J]. Nano Letters, 2003, 3(9): 1235-1238. doi: 10.1021/nl034428i
[13]	SCHOPPLER F, MANN C, HAIN T C, et al. Molar extinction coefficient of single-wall carbon nanotubes[J]. Journal of Physical Chemistry C, 2011, 115(30): 14682-14686. doi: 10.1021/jp205289h
[14]	DOHARE N, SIDDIQUEE M A, AY M P, et al. Esterase activity and interaction of human hemoglobin with diclofenac sodium: A spectroscopic and molecular docking study[J]. Journal of Molecular Recognition, 2020, 33: e2841.
[15]	LAKOWICZ, JOSEPHR. Principles of fluorescence spectroscopy[M]. Plenum Press, 1983.
[16]	张冰卫, 李博, 夏文水, 等. 用荧光光谱法研究分子间结合常数和结合位点数时的公式选择[J]. 药学进展, 2011, 35(007): 296-303.
[17]	MANDAL P, GANGULY T. Fluorescence spectroscopic characterization of the interaction of human adult hemoglobin and two isatins, 1-methylisatin and 1-phenylisatin: A comparative study[J]. Journal of Physical Chemistry B, 2009, 113(45): 14904-14913. doi: 10.1021/jp9062115
[18]	YANG Z, CHENG X, LI X. Investigation of the interaction between human hemoglobin and five antioxidants by fluorescence spectroscopy and molecular modeling[J]. Journal of the Iranian Chemical Society, 2018, 15: 245-257. doi: 10.1007/s13738-017-1227-5