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    王娅君, 赵莉, 徐佳杰, 付水林, 宫衡. 克雷伯氏肺炎杆菌生产1,3-丙二醇代谢途径中的酶活变化[J]. 华东理工大学学报（自然科学版）, 2007, (4): 481-484.
    引用本文: 王娅君, 赵莉, 徐佳杰, 付水林, 宫衡. 克雷伯氏肺炎杆菌生产1,3-丙二醇代谢途径中的酶活变化[J]. 华东理工大学学报（自然科学版）, 2007, (4): 481-484.
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    WANG Ya-jun, ZHAO Li, XU Jia-jie, FU Shui-lin, GONG Heng. Changes in the Activities of Metabolic Enzymes in Klebsiella pneumoniae during 1,3-Propanediol Production[J]. Journal of East China University of Science and Technology, 2007, (4): 481-484.
    Citation: WANG Ya-jun, ZHAO Li, XU Jia-jie, FU Shui-lin, GONG Heng. Changes in the Activities of Metabolic Enzymes in Klebsiella pneumoniae during 1,3-Propanediol Production[J]. Journal of East China University of Science and Technology, 2007, (4): 481-484.
    shu

    克雷伯氏肺炎杆菌生产1,3-丙二醇代谢途径中的酶活变化

    Changes in the Activities of Metabolic Enzymes in Klebsiella pneumoniae during 1,3-Propanediol Production

      摘要
    • 摘要: 考察了不同条件下克雷伯氏肺炎杆菌(Klebsiella.pneumoniae)合成1,3-丙二醇(1,3-PD)的3种关键酶(甘油脱氢酶(GDH)、1,3-PD氧化还原酶(PDOR)及甘油脱水酶(GDHz))的表观酶活变化。结果显示:这3种酶的酶活变化与Klebsiella.pneumoniae的1,3-PD代谢不完全相关。用SDS-PAGE电泳分析上述不同发酵条件下酶活变化,结果显示不同于3种酶的蛋白条带,Mr约为4.0×104位置的蛋白条带有明显的变化,这可能与菌体代谢过程相关,结论有待进一步深入研究。

       

      Abstract: The activities of the three enzymes(glycerol dehydrogenase(GDH),glycerol dehydratase(GDHt),1,3-propanediol oxidoreductase(PDOR)),considered as key metabolic enzymes for 1,3-propanediol formation in Klebsiella.pneumoniae were investigated at different experimental conditions in this work.Results showed that the variety of three enzymes were not completely interrelated with the 1,3-PD production in Klebsiella.pneumoniae.The expression patterns of enzymes under different culture conditions were analyzed by usi...

       

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