丙酸盐引起麦白霉素生产菌株生长与代谢的改变

Alterations in Growth and Metabolism of Meleumycin Producing Strains after Adding Propionate

摘要: 通过在培养基中添加一定质量浓度的丙酸盐，研究其对麦白霉素合成的影响。在发酵培养基中添加质量浓度为6×10-4 g/mL的丙酸盐，不但能使麦白霉素的生物效价提高35.2%，而且能提高其组分中麦迪霉素A1的含量，但是添加丙酸盐对菌体的生长不利。发酵12 h添加丙酸盐，产物的合成受到相当大的抑制，且出现二次生长现象，最终菌浓比对照高出40%。发酵12 h添加丙酸盐对麦白霉素生产菌的影响可能是提高了生长代谢的通量，从而使代谢的产物合成通量降低。

Abstract: In order to investigate the influence of propionate on meleumycin biosynthesis, certain concentration of propionate was added to the fermentation medium. The meleumycin product increased by 35.2% and the synthesis of midecamycin A1 was promoted as the result of 6×10-4 g/mL propionate added at the beginning of fermentation, but the growth of strain was inhibited. The synthesis of meleumycin was repressed and the strain appeared secondary growth when propionate was added at the time of 12 h. The cell concentration was 40% higher than that of control. The promoted growth metabolic flux may be lead to depress the product metabolic flux by addition of propionate at the time of 12 h.