Abstract: The possibility of using in vitro shoot culture to evaluate salt tolerance of different tomato species was determined. Shoot with 3 leaves grew in media supplemented with 0,75,150,300,450 mmol/L NaCl, and rooting and growth were determined. The different degree of salt tolerance among species was clearly shown on the basis of the shoot rooting and growth. Studies on POD isoenzymes at 75mmol/L NaCl stress and without stress were carried out. Exogenous DNA extracted and purified from the leaves of wild tomato species was injected into the ovary of cultivated tomato about 24h after artificial self pollination. Some characters of the donor were observed in offsprings. The results obtained in this paper showed Lycopersicon pennellii LA716 was the most salt tolerant in vitro shoot culture and it could be a better system for testing and selecting for salt tolerance.