Abstract: The chlorination of sucrose-6-acetates (S-6-A) to produce sucralose-6-acetate (TGS-6-A) is a key step in the synthesis of sucralose. In this work, a thermodynamic calculation of the chlorination reaction was carried out using the group contribution method. The results show that the reaction is exothermic and irreversible in the temperature range of 372 K to 389 K. The thermodynamic calculations were performed on the hydrolysis reaction of TGS-6-A. The equilibrium constants at different temperatures obtained agree well with the experimental values, confirming the reliability of the calculation methods used in this work. Then, the effect of temperature on reaction rate was studied by batch experiments, and a chain reaction kinetic model of chlorination reaction was established. The activation energies of the main reactions producing Dichlorosucrose-6-acetic acid ester and TGS-6-A were determined to be 103.87 kJ/mol and 153.87 kJ/mol, respectively, and the activation energy of the side reaction 87.09 kJ/mol. A kinetic study suggests that the main reaction is dependent on reaction temperature, and increasing the temperature and controlling the reaction time can effectively improve the yield of the target product, TGS-6-A.