Abstract:
(S)-2-amino-4-phenylbutyric acid can be prepared by enantioselective hydrolysis of N-phenylacetyl-2-amino-4-phenylbutyric with penicillin G acylase from Alcaligenes faecalis (AfPGA). pH vaule and temperature during the hydrolysis process had no significant influence on enantioselectivity of AfPGA. Compared with water phase, acetic ester with saturated content of water was an ideal reaction system for aforementioned hydrolysis process. Significant promotion in solubility of substrates was observed with acetic ester phase. It was also more convenient to recover the products from acetic ester with saturated content of water. When the substrate conversion rate was 48.6%, the e.e.value of (S)-2-amino-4-phenylbutyric acid achieved 99.8%.