Abstract:
A selection system for hybrids by protoplast fusion between Chaetomium cupreum and C.-globosum using dominant selective drug resistance markers were developed. C.-cupreum was sensitive to G418 (Gs) and resistant to hygromycin B(Hr). C.-globosum was sensitive to both G418 (Gs) and hygromycin B (Hs). Agrobacterium tumefaciens mediated neomycin phosphotransferase II gene transformation of C.-globosum (Gs, Hs) acquired resistance to G418 strains (Gr, Hs). Protoplasts of C.-cupreum (Hr, Gs) and C.-globosum (Hs, Gr) were prepared, mixed in the presence of polyethylene glycol 6000 and incubated on regeneration agar plates. Two regenerated colonies showing Hr and Gr were regarded as hybrids by amplified fragment length polymorphisms analysis of the genomic DNA and they displayed a significant exchange of taxonomic characteristics between the parental strains.