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    丁庆豹, 邱蔚然. 乙酰短杆菌酶法合成5—甲基尿苷[J]. 华东理工大学学报（自然科学版）, 1999, (3): 284-287.
    引用本文: 丁庆豹, 邱蔚然. 乙酰短杆菌酶法合成5—甲基尿苷[J]. 华东理工大学学报（自然科学版）, 1999, (3): 284-287.
    shu
    Enzymatic Synthesis of 5 Methyluridine by Brevibacterium acetylicum[J]. Journal of East China University of Science and Technology, 1999, (3): 284-287.
    Citation: Enzymatic Synthesis of 5 Methyluridine by Brevibacterium acetylicum[J]. Journal of East China University of Science and Technology, 1999, (3): 284-287.
    shu

    乙酰短杆菌酶法合成5—甲基尿苷

    Enzymatic Synthesis of 5 Methyluridine by Brevibacterium acetylicum

      摘要
    • 摘要: 用乙酰短杆菌完整细胞为酶源，以鸟苷和胸腺嘧啶为底物，酶法合成５－甲基尿苷。最佳反应条件为：底物浓度１００－２００ｍｍｏｌ／Ｌ，ｐＨ８．０，磷酸盐缓冲液尝试１０－５０ｍｍｏｌ／Ｌ，６０℃，摇床转速１２０ｒ／ｍｉｎ，反应１ｈ即达到平衡，５－甲基尿苷转化率达６０％以上。

       

      Abstract: Methyluridine was enzymatically produced from guanosine and thymine by intact cells of Brevibacterium acetylicum QD9805. The optimal reaction conditions were researched. When the reaction mixtures, containing 100200mmol/L substrates, 1050mmol/L phosphate buffer(pH 8.0),were incubated at 60C shaking at 120r/min for 1h, the conversion yield could reach above 60%.

       

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