Abstract:
Marine-derived filamentous fungus
Aspergillus glaucus HB1-19 is sensitive to mechanical shear stress and thus causes great difficulties in bioreactor fermentation. UDP-galactofuranose transporter (UgtA) and UDP-glucose epimerase (UgeA) play critical roles in wall formation. In this work, we aim to study their functions in cell wall synthesis and also their relationships with shear-sensitivity. Degenerate PCR and genome walking were used to obtain the nucleotide sequences of
AgugtA and
AgugeA.
AgugtA is 1 377 bp containing four introns locating at 135-192, 261-327, 552-601, 1 047-1 096 bp separately, and codes a protein of 383 amino acids.
AgugeA is 1 322 bp containing three introns locating at 30-116, 352-420, 878-927 bp separately, and codes a protein of 371 amino acids. Phylogenetic analysis suggested that AgugtA and AgugeA are evolutionally conserved and probably possess the same effects to their homologs in
Aspergillus spp. Finally, deletion attampts of
AgugeA genetically or inhibition of AgugeA at the protein level severely suppressed the growth of
Aspergillus glaucus HB1-19, indicating that
AguaeA is essential for HB1-19, which could be closely related with its high shear sensitivity.