Abstract: The dead time of reversed-phase high performance liquid chromatography isdetermined bv the iterative calculation method on the basis of retention times of two kinds ofhomologues,nonpolar n-alkanes and weak polar n-alkylbenzenes,as well as by the use of u-racil and D_2O.It is found that the both methods provide reasonably good estimates of thedead time,The dead times calculated by the retention times of a homologues series of alkyl-benzenes have slightly greater values than that calculated by alkanes.A listing of the C language program ls given.