Abstract: Tetra O acetyl 1 allylidiene D glycosides were synthesized by chemical, ultrasonic chemical and photochemical methods respectively and the results showed ultrasonic method was the most efficient one. ESR technique and correspondent experiments proved that the reaction to produce this kind of glycosides was an ionic elimination reation. The deacetylated products were tested for their pharmacological activity and were found having in vitro immunological adjustment effect on T,B lymphocyte.