Abstract: Citric acid is one of the most versatile industrial organic acids in beverages, pharmaceutical, cosmetics, agriculture and biomedicine. Citric acid production using corn stover biomass as feedstock could provide an important way for cost reduction. The technical level of the reported citric acid production from lignocellulose biomass is far from the practical industry application. In this study, Aspergillus niger SIIM M288 was used to produce high titer citric acid using the corn stover hydrolysate as the substrate. Under the optimized condition, the citric acid titer and yield were achieved at 97.45 g/L and 87.27%, respectively, by Aspergillus niger SIIM M288 using corn stover hydrolysate. The results reached the highest level up to now. The results provided a possibility for economically citric acid production using the alternative lignocellulose feedstock.