胰高血糖素样肽1受体N端片段与exendin-4的亲和位点分析

高蔚丰; 刘美云; 马昱澍

高蔚丰, 刘美云, 马昱澍. 胰高血糖素样肽1受体N端片段与exendin-4的亲和位点分析[J]. 华东理工大学学报（自然科学版）, 2008, (1): 66-70.

引用本文:

高蔚丰, 刘美云, 马昱澍. 胰高血糖素样肽1受体N端片段与exendin-4的亲和位点分析[J]. 华东理工大学学报（自然科学版）, 2008, (1): 66-70.

GAO Wei-feng, LIU Mei-yun, MA Yu-shu. Analysis of the Binding Site of the N-Terminal Fragment of Glucogan-Like Peptide-1 Receptor to exendin-4[J]. Journal of East China University of Science and Technology, 2008, (1): 66-70.

Citation:

GAO Wei-feng, LIU Mei-yun, MA Yu-shu. Analysis of the Binding Site of the N-Terminal Fragment of Glucogan-Like Peptide-1 Receptor to exendin-4[J]. Journal of East China University of Science and Technology, 2008, (1): 66-70.

胰高血糖素样肽1受体N端片段与exendin-4的亲和位点分析

Analysis of the Binding Site of the N-Terminal Fragment of Glucogan-Like Peptide-1 Receptor to exendin-4

摘要

摘要: 通过易错PCR(epPCR)方法建立了一个鼠肺的胰高血糖素样肽1受体(GLP-1R)N端片段的噬菌体随机突变展示肽库.根据筛选出的突变体来分析突变后胰高血糖素样肽1受体N端片段(nGLP-1R)与exendin-4结合活性.实验结果显示:保守的半胱氨酸C26发生突变后并未引起nGLP-1R与其配体exendin-4结合能力的改变,第101位和108位氨基酸是nGLP-1R与exendin-4结合的潜在位点.

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