Abstract:
A basic peptide derived from human immunodeficiency virus HIV1 TAT peptide (positions 47-57) can translocate through the cell membranes, the characteristics of which are utilized for the delivery of exogenous proteins into cells. The coding sequence for the TATapoptin was amplified by polymerase chain reaction(PCR), then inserted into pET28b vector and highly expressed in Escherichia coli BL21(DE3). The protein expressed in inclusion body was purified by NiNTA under denaturing condition. Apoptosis activity of the TAT apoptin was evaluated by MTT. Results indicated that HeLa cells were highly sensitive to the purified TAT apoptin.