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    李新月, 叶蕊芳, 郑黎, 郑一涛, 李晴, 薛建萍. Arthrobacter globiformis酯酶基因的克隆、表达和酶活性测定[J]. 华东理工大学学报(自然科学版), 2006, (8): 930-932979.
    引用本文: 李新月, 叶蕊芳, 郑黎, 郑一涛, 李晴, 薛建萍. Arthrobacter globiformis酯酶基因的克隆、表达和酶活性测定[J]. 华东理工大学学报(自然科学版), 2006, (8): 930-932979.
    LI Xin-yue, YE Rui-fang, ZHENG Li, ZHENG Yi-tao, LI Qing, XUE Jian-ping. Cloning and Expression of the Esterase Gene of Arthrobacter globiformis and the Activity Assay of the Esterase[J]. Journal of East China University of Science and Technology, 2006, (8): 930-932979.
    Citation: LI Xin-yue, YE Rui-fang, ZHENG Li, ZHENG Yi-tao, LI Qing, XUE Jian-ping. Cloning and Expression of the Esterase Gene of Arthrobacter globiformis and the Activity Assay of the Esterase[J]. Journal of East China University of Science and Technology, 2006, (8): 930-932979.

    Arthrobacter globiformis酯酶基因的克隆、表达和酶活性测定

    Cloning and Expression of the Esterase Gene of Arthrobacter globiformis and the Activity Assay of the Esterase

    • 摘要: 采用聚合酶链反应(PCR)的方法扩增了A rthrobacter g lobiform is中酯酶编码基因,与载体质粒连接后在大肠杆菌BL 21(DE 3)中进行表达。以包涵体形式表达的蛋白在8 m o l/L尿素作用下溶解,经过透析复性后获得了具有活性的粗蛋白。产物活性实验表明,酯酶表现出较高的催化活性,为菊酯作为杀虫剂的应用奠定了基础。

       

      Abstract: A novel esterase which stereoselectively hydrolyzes the( )-trans stereoisomer of ethyl chrysanthemate to produce( )-trans-chrysanthemic acid was discussed.The gene coding for the esterase was amplified from Arthrobacter globiformis by polymerase chain reaction(PCR).The gene was inserted into a vector and expressed in Escherichia coli BL21(DE3).The protein expressed in body was dissolve in 8 mol/L urea before dialysis.The purified crude enzyme show high catalysis activity.It may suggest the possibility that( )-trans-chrysanthemate is identified as the insecticide.

       

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