Abstract:
In this study, effects of aromatic amino acids and vitamins on the growth of transketolase-deficient Bacillus subtilis and D-ribose biosynthesis were studied in chemically defined media. It was found that aromatic amino acids, biotin, pyridoxine and nicotinic acid promoted cell growth, and nicotinic acid enhanced D-ribose production significantly. The response surface methodology (RSM) was applied to optimize the concentration of vitamins in the minimal medium. Under optimal condition, the production of D-ribose was enhanced to (23.4±0.39) g/L, which is about 2.31-fold compared with the case without vitamins supplementation, and the molar yield based on glucose reached 0.353 mol/mol.