Abstract: Chemically synthesized racemic naproxen was resolved by biological method and optically pure S enantiomer was prepared. Lipase from Candida cylindracea was able to catalyze the hydrolysis of methyl, ethyl and ethoxyethanol naproxen ester effectively and enantioselectively. S enatiomer was obtained by separation from remained ester. As a carrier, HZ 806, the porous adsorption resin with middle polarity can make a micro circumstance helpful to hydrolysis around enzyme molecules. Enzyme activity was improved and enzyme could be recycled easily with immobilization on HZ 806. Racemic ethoxyethanol naproxen ester could be resolved by hydrolyzing continuously in packed bed reactor with lipase immobilized on HZ 806. S naproxen with 89.1% optical purity was obtained.