Abstract:
The biosynthesis of clavulanic acid (CA) is significantly influenced by different kinds of organic nitrogen sources. In the present study, the effects of amino acid composition of different nitrogen sources on CA biosynthesis were analyzed by cluster analysis method. The result showed that aspartic acid, glutamic acid, threonine, lysine and methionine were the key factors affecting clavulanic acid production. The production of clavulanic acid increased remarkably with the supplement of aspartic acid, glutamic acid, threonine and lysine, which increased by 22.3%、39.3%、16.3% and 6.3%, respectively. However, the addition of methionine had a strong inhibitory effect on it(the production reduced by 23.3%).