Abstract: Comparing skeletal muscle with pneumatic muscle actuator, the mechanical characteristic of pneumatic muscle actuator was similar to that of skeletal muscle. The pneumatic muscle actuator was used as dynamic element. The hardware and software of control and measurement systems were designed and developed. The engineered tendon was constructed with the tendon bioreactor. The experimental results on tissue engineered tendon indicated that running of this newly designed tendon bioreactor was stable, convenient and safe. Tissue engineered tendon could be constructed for up to 12 weeks.