Abstract: A novel method is broposed for obtaining a chromatographic plot with well-separated peaks in quantitative analysis with HPLC automatically, and thus a prospect is expected for intelligent computerization in such a variety of instruments. To improve the separation condition, the ultilization of the new mulfisegment gradient elution technique is adopted, it will naturally tend to increase the complexily of the searching work. To overcome this difficulty, the search work is handed over to a microcomputer searching the satisfactory separation condition heuristically rather than searching the optimal one, because the former is more rational and convenient. It has been applied with success to the analysis of 21 primary amino acids in human blood plasma, and some other research work of ours are also presented here briefly.