一株真菌所产环缩酚酸肽类化合物的分离和鉴定

Isolation and Structural Elucidation of Cyclodepsipeptides from a Fungal Strain

摘要: 对一株真菌ZME及其所产的次级代谢产物进行分离鉴定。通过形态观察和ITS测序对真菌ZME进行菌株鉴定，并对其发酵液上清液和菌丝体分别用乙酸乙酯和甲醇萃取；萃取后的有机试剂粗提物经硅胶柱层析和半制备HPLC分离纯化；纯化后的各组分利用光谱分析进行结构解析。结果表明，真菌ZME初步判断属于Beauveria felina。对这株真菌次级代谢物结构解析，共鉴定5个化合物Isaridin B、Isaridin A、Roseocardin、Roseotoxin B和Destruxin B，这5个化合物均为环缩酚酸肽类化合物，其中Roseocardin具有抗细菌活性。

Abstract: Abstract: The study is to isolate and elucidate the structure of secondary metabolites produced by fungal strain ZME. Strain ZME was identified according to its morphology and sequence. Ethyl acetate and methanol were used to extract fermentation broth and mycelium of strain ZME, respectively. The extract was isolated and purified by silica gel column chromatography and semi preparative HPLC. Chemical structures of the purified fractions were elucidated by spectroscopic analysis. Results show that strain ZME belongs to Beauveria feline. Five compounds are isolated from the extract and their structures are elucidated as Isaridin B, Isaridin A, Roseocardin, Roseotoxin B and Destruxin B. These five compounds are cyclodepsipeptides, among which Roseocardin has antibacterial activity.