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    王然明, 王泽建, 田锡炜, 张云飞, 王永红, 储炬. 生物过程尾气质谱仪在乳酸发酵工艺控制中的应用[J]. 华东理工大学学报(自然科学版), 2013, (3): 289-295.
    引用本文: 王然明, 王泽建, 田锡炜, 张云飞, 王永红, 储炬. 生物过程尾气质谱仪在乳酸发酵工艺控制中的应用[J]. 华东理工大学学报(自然科学版), 2013, (3): 289-295.
    WANG Ran-ming, WANG Ze-jian, TIAN Xi-wei, ZHANG Yun-fei, WANG Yong-hong, CHU Ju. Application of Exhausting Gas Mass Spectrometer in Metabolic Regulation of Lactic Acid Fermentation[J]. Journal of East China University of Science and Technology, 2013, (3): 289-295.
    Citation: WANG Ran-ming, WANG Ze-jian, TIAN Xi-wei, ZHANG Yun-fei, WANG Yong-hong, CHU Ju. Application of Exhausting Gas Mass Spectrometer in Metabolic Regulation of Lactic Acid Fermentation[J]. Journal of East China University of Science and Technology, 2013, (3): 289-295.

    生物过程尾气质谱仪在乳酸发酵工艺控制中的应用

    Application of Exhausting Gas Mass Spectrometer in Metabolic Regulation of Lactic Acid Fermentation

    • 摘要: 将生物过程尾气分析质谱仪及相应的Biostar处理软件成功应用于乳酸发酵过程中,通过实时监测发酵过程中不同阶段和不同发酵条件下菌体生理代谢参数的动态变化,并对其进行多参数相关分析,阐明了发酵过程中杂质组成的合成代谢过程;利用尾气分析质谱仪进行微耗氧发酵过程供氧水平的精确控制,当菌体的摄氧率控制在0.70 mmol /(L·h)时,凝结芽孢杆菌乳酸的最佳合成速率能达到2.78 g/(L·h);同时在以玉米淀粉水解糖液为基质的乳酸发酵过程中,通过对菌体生理代谢参数变化与残糖的消耗和副产物生成过程进行相关分析,建立了根据生理参数的变化进行发酵过程终点快速判定方法,实现了L 乳酸发酵产量和质量的在线控制策略。

       

      Abstract: Lactic acid, as a widely occurring hydroxycarboxylic acid, plays important roles in food, pharmaceutical, textile, leather and chemical industries. In this work, exhausting gas mass spectrometer was successfully introduced into lactic acid fermentation. Furthermore, combining the dynamic changes of macroscopic physiological parameters and fermentation conditions, the prime parameters could be determined under various states through multi parameters relevant analysis method. The fermentation process of impurities in the synthesis of metabolic process was illuminated. Based on exhausting gas analysis by process mass spectrometer for micro aerobic fermentation process, the oxygen supply levels were accurately controlled and optimized; when the the oxygen consumption rate was controlled at 0.70 mmol/(L·h), the highest L lactic acid production rate reached 2.78 g/(L·h). At the same time,through the correlation analysis of physiological parameters, residual sugar consumption and by products formation under the matrix medium of corn starch hydrolysis sugar solution, we established the fermentation process fast on line control method based on physiological parameters dynamic changes, and realized the yield and quality online control strategy of L lactic acid fermentation.

       

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