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    朱智东, 唐燕发, 等. 底物诱导和分批补料对巨大芽孢杆菌环氧化物水解酶生物合成的影响[J]. 华东理工大学学报（自然科学版）, 2001, (3): 243-246.
    引用本文: 朱智东, 唐燕发, 等. 底物诱导和分批补料对巨大芽孢杆菌环氧化物水解酶生物合成的影响[J]. 华东理工大学学报（自然科学版）, 2001, (3): 243-246.
    shu
    Effect of Substrate Induction and Fed-batch Operation on Biosynthesis of Epoxide Hydrolase from Bacillus megaterium ECU1001[J]. Journal of East China University of Science and Technology, 2001, (3): 243-246.
    Citation: Effect of Substrate Induction and Fed-batch Operation on Biosynthesis of Epoxide Hydrolase from Bacillus megaterium ECU1001[J]. Journal of East China University of Science and Technology, 2001, (3): 243-246.
    shu

    底物诱导和分批补料对巨大芽孢杆菌环氧化物水解酶生物合成的影响

    Effect of Substrate Induction and Fed-batch Operation on Biosynthesis of Epoxide Hydrolase from Bacillus megaterium ECU1001

      摘要
    • 摘要: 从土壤中筛选得到的巨大芽孢杆菌（Bacillus megaterium)ECU1001所产环氧化物水解酶能高度对映选择性地水解外消旋缩水甘油苯基醚，在摇瓶和5L发酵罐中培养时初始产酶水平分别为11．0U／L和31．0U／L，通过添加底物诱导，可使摇瓶培养中酶量达到61．0U／L，在5L发酵罐中通过补料分批发酵，酶活可进一步提高到95．6U／L。

       

      Abstract: An epoxide hydrolase biosynthesized by Bacillus megaterium EUC1001 isolated from soil can hydrolyze racemic phenyl glycidyl ether (PGE) with high enantioselectivity. Initial activities of the epoxide hydrolase obtained in flask and 5L fermentor were 11.0U/L and 31.0U/L, respectively. The enzyme activity in flask reached 61.0U/L by adding the substrate as an inducer and it could even reach 96.6U/L through fed batch fermentation in a 5L fermentor.

       

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