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    杨善祥，, 钱秀珍，, 王丽丹，, 徐云龙. 羧甲基壳聚糖修饰阿霉素纳米脂质体的制备及体外细胞实验[J]. 华东理工大学学报（自然科学版）, 2012, (2): 181-185.
    引用本文: 杨善祥，, 钱秀珍，, 王丽丹，, 徐云龙. 羧甲基壳聚糖修饰阿霉素纳米脂质体的制备及体外细胞实验[J]. 华东理工大学学报（自然科学版）, 2012, (2): 181-185.
    shu
    YANG Shanxiang，, QIAN Xiuzhen，, WANG Lidan，, XU Yunlong. Preparation of pHSensitive Doxorubicin Liposomes Modified with Carboxymethyl Chitosan and in Vitro Cell Research[J]. Journal of East China University of Science and Technology, 2012, (2): 181-185.
    Citation: YANG Shanxiang，, QIAN Xiuzhen，, WANG Lidan，, XU Yunlong. Preparation of pHSensitive Doxorubicin Liposomes Modified with Carboxymethyl Chitosan and in Vitro Cell Research[J]. Journal of East China University of Science and Technology, 2012, (2): 181-185.
    shu

    羧甲基壳聚糖修饰阿霉素纳米脂质体的制备及体外细胞实验

    Preparation of pHSensitive Doxorubicin Liposomes Modified with Carboxymethyl Chitosan and in Vitro Cell Research

      摘要
    • 摘要: 以羧甲基壳聚糖（CMCT）为修饰剂，采用薄膜pH梯度法制备具有pH敏感性的阿霉素纳米脂质体（CMCTDOXNL），以增加抗癌药物在肿瘤部位的蓄积，同时增强抗癌药物向肿瘤细胞内的传递。结果表明：制备的CMCTDOXNL粒子形貌圆整，粒径分布均匀为 （38±221）nm，药物包封率为8883%；相比传统的阿霉素纳米脂质体（DOXNL）， CMCTDOXNL与Hela细胞的结合和摄取均有所提高，对细胞的杀伤作用更强； CMCTDOXNL的体外药物释放具有明显的pH敏感性，比普通的阿霉素脂质体更能促进阿霉素（DOX）向肿瘤细胞内的传递。

       

      Abstract: To enhance the accumulation in the tumor and intracellular delivery of the encapsulated doxorubicin(DOX), a pHsensitive doxorubicin nanoliposomes(DOXNL) modified with carboxymethyl chitosan(CMCT) was prepared by thinfilm pH gradient. The nanoliposomes modified with CMCT showed good shape with an average particle size of (38±221) nm and the drug encapsulation efficiency was about 8883%. The drug release of the CMCTmodified DOX nanoliposomes in vitro showed obvious pH sensitivity. The Hela cells treated with CMCTDOXNL showed remarkable increase in cellular association or DOX uptake compared with DOXNL. The cytotoxicity of CMCTDOXNL on Hela cells was higher than that of DOXNL. The pHsensitive CMCTmodified DOX nanoliposomes promoted the delivery of the encapsulated DOX into the tumor cells.

       

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