种球溶胀法制备有机聚合物色谱填料及其在纯化红景天苷中的应用
Preparation of Organic Polymer Chromatographic Packings by Swelling Seeds and Their Application for Seperation of Salidroside
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摘要: 采用分散聚合法,以正丁醇为介质制成单分散的聚苯乙烯微球,应用该微球通过种球溶胀法制备聚苯乙烯-二乙烯苯(PSt-DVB)色谱填料。应用高压匀质机制备的活化种球和溶胀活化种球的乳液更稳定,液滴更小。该填料单分散性能优越,填装4.6 mm×150 mm的色谱柱背压低,可用作分析色谱和制备色谱。该色谱柱用于红景天苷的分离,可获得98%以上的纯品。Abstract: Monodisperse polystyrene particles as the swollen seeds for synthesizing macroporous(poly(styrene-)co-divinylbenzene) organic polymer chromatographic packings were prepared by dispersion polymerization in n-butanol reaction media.And the stable fine emulsions for swelling seeds could be obtained by using a high-pressure homogenizer.The chromatographic packings exhibit excellent monodispersibility and low back pressure while packed into chromatographic column,and can be employed for the separation and purifi...