Abstract: In search for more reasonable process for preparation of polyfluorated quinolone antibacterial agents, temafloxacin hydrochloride is prepared from ethyl 2,4 dichloro 5 fluorobenzoylacetate, via condensation, amination, cyclization, boron esterification, substitution, hydrolysis and salt formation with 45% overall yield. The structure of temafloxacin hydrochloride is confirmed by MS, 1H NMR, IR and EA.