Abstract: A novel kind of guided tissue regeneration (GTR) barrier membrane was fabricated using an immersion-precipitation phase inversion technique with the biodegradable material of chitosan. The morphology of the prepared membrane was described by scanning electronic microscopy. The effects of polymer concentration and solvent evaporation time on the porosity, average pore size, swelling ratio and the mechanical properties as tensile strength and elongation of the membrane were also investigated. (Research) results show that the membrane has an asymmetric structure, which consists of a dense skin layer, a thin transition region and a sponge like porous layer. Polymer concentration and solvent evaporation time are two key factors that have great effect on the structure and the properties of the membrane. Both a bigger polymer concentration and a longer solvent evaporation time result in a lower porosity and a smaller swelling ratio, while tensile strength of the membranes are raised at the same time. This work may be help for the development of a novel kind of GTR barrier membrane material.