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    何婉瑛, 张立国, 倪力军. 大鼠口服灌胃腰痛宁粉的药物代谢动力学[J]. 华东理工大学学报(自然科学版), 2014, (4): 444-448.
    引用本文: 何婉瑛, 张立国, 倪力军. 大鼠口服灌胃腰痛宁粉的药物代谢动力学[J]. 华东理工大学学报(自然科学版), 2014, (4): 444-448.
    HE Wan-ying, ZHANG Li-guo, NI Li-jun. Pharmacokinetics of Yaotongning Capsule after Intragastric Administration in Rats[J]. Journal of East China University of Science and Technology, 2014, (4): 444-448.
    Citation: HE Wan-ying, ZHANG Li-guo, NI Li-jun. Pharmacokinetics of Yaotongning Capsule after Intragastric Administration in Rats[J]. Journal of East China University of Science and Technology, 2014, (4): 444-448.

    大鼠口服灌胃腰痛宁粉的药物代谢动力学

    Pharmacokinetics of Yaotongning Capsule after Intragastric Administration in Rats

    • 摘要: 采用高效液相色谱(HPLC)技术检测大鼠血浆中马钱子碱和士的宁的血药浓度,并研究了大鼠口服灌胃腰痛宁粉悬浊液后的药物代谢动力学行为。结果显示:不同质控浓度马钱子碱和士的宁的回收率均高于60%,日内及日间精密度相对标准偏差(RSD)均低于15%,稳定性及线性关系良好,满足生物样品定量分析要求;大鼠单剂量(800 mg/kg)灌胃给予(intragastric administration)腰痛宁后,马钱子碱和士的宁的代谢均符合二室开放模型;与士的宁相比,马钱子碱吸收较慢,消除较快,生物利用度较低。本法简便快捷、专属性强、结果准确,适于腰痛宁的药物代谢动力学行为研究。

       

      Abstract: A feasible assay method to determine brucine and strychnine in rat plasma by high performance liquid chromatography (HPLC) was developed. The pharmacokinetics of yaotongning capsule after intragastric administration in rats was studied. Results showed that the recovery of brucine and strychnine in different quality control concentrations surpassed 60%, intra day and inter day RSD (Relative Standard Deviation) were lower than 15%, stability and linearity were fine enough to meet the requirements of bioanalytical method. The kinetic processes of brucine and strychnine were all in accordance with two compartment open model after intragastric administration yaotongning capsule; Brucine assimilated slower, eliminated faster and had inferior bioavailability than strychnine. The method is convenient and reliable. It has good repeatability, precision, and stability. It can be applied to study the pharmacokinetics of yaotongning capsule.

       

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