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    张立, 金亦辉, 张元兴. 在无血清培养基中代谢副产物对杂交瘤细胞生长代谢的影响 I.乳酸的作用[J]. 华东理工大学学报(自然科学版), 2002, (2): 157-160.
    引用本文: 张立, 金亦辉, 张元兴. 在无血清培养基中代谢副产物对杂交瘤细胞生长代谢的影响 I.乳酸的作用[J]. 华东理工大学学报(自然科学版), 2002, (2): 157-160.
    Influence of Metabolic Byproducts on Growth and Metabolism of Hybridoma Cells in Serum-free CulturesI. Effect of Lactate[J]. Journal of East China University of Science and Technology, 2002, (2): 157-160.
    Citation: Influence of Metabolic Byproducts on Growth and Metabolism of Hybridoma Cells in Serum-free CulturesI. Effect of Lactate[J]. Journal of East China University of Science and Technology, 2002, (2): 157-160.

    在无血清培养基中代谢副产物对杂交瘤细胞生长代谢的影响 I.乳酸的作用

    Influence of Metabolic Byproducts on Growth and Metabolism of Hybridoma Cells in Serum-free CulturesI. Effect of Lactate

    • 摘要: 研究了无血清批培养中乳酸对杂交瘤细胞生长代谢的影响,当乳酸浓度低于5.3mmol/L时,乳酸对细胞生长和代谢无明显影响,当乳酸浓度在10mmol/L-25mmol/L时,对杂交瘤细胞的生长代谢有弱抑制,当乳酸浓度高于34mmol/L时,对细胞生长代谢有较明显的抑制。

       

      Abstract: In the batch cultures of WuT3 hybridoma cells in serum free medium with different lactate concentrations, the influences of lactate on cells growth and metabolism were investigated. It was found that lactate below 5.3mmol/L influenced hardly on cells, lactate between 10mmol/L and 25mmol/L inhibited a little on cells growth and metabolism, and lactate above 34mmol/L inhibited strongly on cell cultures.

       

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