Abstract: The puberty of offspring was affected by maternal effects. In order to further study the action mechanism of maternal effect, the inbred strain of C57BL/6J mouse was selected to establish the experimental model. By using vaginal opening (VO) of female mice and balano preputial separation (BPS) of male mice as the signal of puberty timing, the effect of maternal luteinizing hormone (LH) level on puberty timing of the offspring was studied. Puberty timing of the male offspring of treatment group is slower than that of control group (P < 0.010), and the weights of the two groups on the onset time have significant difference. Puberty timing of the female offspring of treatment group is faster than that of the control group (P < 0.002), and the weights of the two groups on the onset time have no significant difference. It is suggested that the maternal LH level has significant impact on puberty timing of mice, but in different ways when offspring mice have gender difference.