Abstract: GD-46 Macroreticulor Polymer adsorbent which belongs to the series of styrene-divinylbenzene polymers was developed in our laboratory. It has high adsorption capacity for active bio-substances, Such as Gibberellic Acid (920), Spiramycin etc., because of its apparent non-polarity, large specific surface area and suitable aperture. The way of preparation and physical properties of GD-46 macroreticular polymer adsorbent were studied. The tests for adsorption of Gibberellic Acid and Spiramycin were accomplished. The results showed that the adsorption capacities for above-mentioned substances are 4% (static) and 10.5% (dynamic) higher than Diaion HP-20 (made in Japan), and the performance of selective adsorption is also superior to Diaion HP-20, so that GD-46 Macroreticular polymer is an excellent adsorbent to separate such active bio-substances as Gibberellic Acid (920), Spiramycin from fermentation broth.