Abstract:
Resveratrol, a secondary metabolite of stilbene, is synthesized by the L-Phenylalanine pathway in plants. Under external conditions, various plants are used to produce resveratrol. However, the most commonly used plant cell cultures for the production of stilbene are still from grapes. In this paper, an optimal technology for the production of resveratrol from suspension cell of
Vitis vinifera Loes. was established. In response to cell growth and resveratrol biosynthesis, the effects of different carbon sources, nitrogen sources, precursors, inducers and hormones on resveratrol synthesis in the grape cells were studied by single factor test, Plackett-Burman test and response surface methodology. The results showed that the optimal nutrient conditions of suspension cells were as follows: B5 medium 5 g/L, sucrose 37.5 g/L, yeast extract 900 mg/L, phenylalanine 100 mg/L, methyl jasmonate 84 μmol/L, 2, 4-chlorophenoxyacetic acid 4.1 mg/L, 6-benzylaminopurine 0.2 mg/L, polyvinylpyrrolidone 1.0 g/L. Under these conditions, the highest expression of resveratrol was (3026.64 ± 56)μg/g in cell suspension culture at 26 °C for 7 d, which was 51.13 times higher than that before optimization, the optimal nutrient conditions can significantly promote the synthesis of resveratrol. In this study, the nutrient composition of suspension cells of
Vitis vinifera was studied in details, and the optimal culture conditions for the growth and resveratrol synthesis of the suspension cells were determined. On the one hand, it provides a basis for analyzing the function of grape suspension cells, and on the other hand, it lays a good foundation for scale-up the production of resveratrol and other substances.