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    刘畅, 陈阳, 田锡炜, 庄英萍, 储炬, 王泽建. 烷烃为底物合成槐糖脂发酵过程供氧控制优化[J]. 华东理工大学学报(自然科学版), 2022, 48(2): 194-202. DOI: 10.14135/j.cnki.1006-3080.20210221001
    引用本文: 刘畅, 陈阳, 田锡炜, 庄英萍, 储炬, 王泽建. 烷烃为底物合成槐糖脂发酵过程供氧控制优化[J]. 华东理工大学学报(自然科学版), 2022, 48(2): 194-202. DOI: 10.14135/j.cnki.1006-3080.20210221001
    LIU Chang, CHEN Yang, TIAN Xiwei, ZHUANG Yingping, CHU Ju, WANG Zejian. Optimization of Oxygen Supply Control During the Fermentation of Sophorolipids Synthesis from Alkane as Substrate[J]. Journal of East China University of Science and Technology, 2022, 48(2): 194-202. DOI: 10.14135/j.cnki.1006-3080.20210221001
    Citation: LIU Chang, CHEN Yang, TIAN Xiwei, ZHUANG Yingping, CHU Ju, WANG Zejian. Optimization of Oxygen Supply Control During the Fermentation of Sophorolipids Synthesis from Alkane as Substrate[J]. Journal of East China University of Science and Technology, 2022, 48(2): 194-202. DOI: 10.14135/j.cnki.1006-3080.20210221001

    烷烃为底物合成槐糖脂发酵过程供氧控制优化

    Optimization of Oxygen Supply Control During the Fermentation of Sophorolipids Synthesis from Alkane as Substrate

    • 摘要: 以正十六烷为疏水底物考察了不同供氧水平对槐糖脂合成代谢的影响。通过对发酵过程中的代谢通量分布和关键酶活性的整合分析,发现供氧水平显著影响细胞脂肪酸和羟基脂肪酸的合成,同时显著影响葡萄糖和烷烃的利用程度。在线生理参数呼吸商(RQ)能够很好地表征胞内代谢通量的变化,后续有望成为过程调控的关键参数并可以进一步应用于过程优化。最后,在槐糖脂合成期提供合适的供氧水平,不但能够有效提升相对昂贵底物烷烃的利用率,而且能够显著降低功率输入,从而在两个方面提高生产经济性。本文研究结果同样适用于其他菜籽油、油酸等疏水性底物的槐糖脂发酵过程,并为工业规模槐糖脂发酵过程控制策略的开发和应用提供坚实的理论基础。

       

      Abstract: Sophorolipid (SL) is one of the most promising biosurfactants widely used in cosmetics, petroleum, pharmaceutical and other industrial fields. In this study, n-hexadecane was used as a hydrophobic substrate to investigate the effect of different oxygen supply levels on SLs synthesis. Through integrated analyses of metabolic flux distribution and key enzyme activities during the fermentation process, it was found that the oxygen supply level significantly affected the syntheses of fatty acids and hydroxyl fatty acids, and, simultaneously, caused significant changes in the utilization of glucose and alkanes. The on-line physiological parameter respiratory quotient (RQ) well dictated the changes of intracellular metabolic flux, which could act as a key process parameter to regulate cell metabolism. Finally, by enabling an appropriate oxygen supply level during the synthesis period of SLs, the utilization rate of the relatively expensive alkane substrate could be optimized, and the related power input reduced, thus improving the production economy. The results of this study could be extended to the study of other hydrophobic substrates such as rapeseed oil and oleic acid for SLs fermentation, and provided a theoretical basis for the control of industrial-scale SLs fermentation process.

       

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