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    肖颖, 吴梦琪, 张文清, 徐志珍, 夏玮. 茯苓多糖HPLC指纹图谱与免疫活性的相关分析[J]. 华东理工大学学报(自然科学版), 2020, 46(5): 672-679. DOI: 10.14135/j.cnki.1006-3080.20190629003
    引用本文: 肖颖, 吴梦琪, 张文清, 徐志珍, 夏玮. 茯苓多糖HPLC指纹图谱与免疫活性的相关分析[J]. 华东理工大学学报(自然科学版), 2020, 46(5): 672-679. DOI: 10.14135/j.cnki.1006-3080.20190629003
    XIAO Ying, WU Mengqi, ZHANG Wenqing, XU Zhizhen, XIA Wei. Correlation Analysis between HPLC Fingerprint of Polysaccharides from Poria Cocos and Immunological Activity[J]. Journal of East China University of Science and Technology, 2020, 46(5): 672-679. DOI: 10.14135/j.cnki.1006-3080.20190629003
    Citation: XIAO Ying, WU Mengqi, ZHANG Wenqing, XU Zhizhen, XIA Wei. Correlation Analysis between HPLC Fingerprint of Polysaccharides from Poria Cocos and Immunological Activity[J]. Journal of East China University of Science and Technology, 2020, 46(5): 672-679. DOI: 10.14135/j.cnki.1006-3080.20190629003

    茯苓多糖HPLC指纹图谱与免疫活性的相关分析

    Correlation Analysis between HPLC Fingerprint of Polysaccharides from Poria Cocos and Immunological Activity

    • 摘要: 通过高效液相色谱(HPLC) 图建立16 批不同产地茯苓多糖的指纹图谱,以NO 释放浓度为免疫活性指标,测定16 批茯苓多糖刺激处于对数生长期的小鼠巨噬细胞产生的NO 的浓度,并利用正交偏最小二乘分析,研究茯苓多糖单糖组成与免疫活性间的相关性。结果表明:茯苓多糖由葡萄糖、半乳糖、甘露糖、岩藻糖、氨基葡萄糖、葡萄糖醛酸、木糖以及阿拉伯糖等8 种单糖组成;用不同浓度茯苓多糖刺激小鼠巨噬细胞,NO 释放浓度随着茯苓多糖浓度的增大而增大,且均高于空白对照组;茯苓多糖中各单糖对小鼠巨噬细胞释放NO 的浓度均有相关性,半乳糖、阿拉伯糖、甘露糖及葡萄糖对小鼠巨噬细胞的免疫活性呈正相关,其中具有显著性贡献的是葡萄糖、半乳糖和甘露糖。

       

      Abstract: Poria cocos, called FuLing in Chinese, is a traditional Chinese medicinal herb. Poria cocos polysaccharides (PCPs) were extracted from Poria cocos collected from different origins through water extraction and alcohol precipitation. High performance liquid chromatography (HPLC) was applied to establish the fingerprint for 16 batches PCPs. A macrophage cell line RAW274.7 was incubated in different concentrations of 16 batches PCPs. The immunological activity was evaluated based on the proliferation activity of cells as determined by MTT assay and NO production. The immunological activity of 16 batches PCPs were introduced into orthogonal partial least squares analysis to investigate the correlation between monosaccharide compositions and immunological activity. The results showed that PCPs were composed of eight monosaccharides, which were Glc, Gal, Man, Fuc, GlcN, GlcA, Xyl and Ara, respectively. The similarity analysis showed that the similarity of the 16 batches PCPs ranged from 0.980 to 1.000. All the 16 PCPs showed well immunological activities, according to the in vitro activity evaluations. Mouse macrophages were stimulated with different concentrations of PCPs. NO production increased with the increase of PCPs concentration, which was higher than that of the blank. According to the orthogonal partial least squares analysis, all the monosaccharides from PCPs had relationship with immunological activity. Gal, Ara, Man and Glc were positively correlated with the immunological activity of mouse macrophages, while Fuc, GlcA, GlcN and Xyl were negative correlated with the immunological activity of mouse macrophages. Particularly, Glc, Gal and Man in PCPs significantly contributed to the NO production from mouse macrophages.

       

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