Abstract:
Nemadectin produced by
Streptomyces cyaneogriseus ssp.
noncyanogenus (
S.
cyaneogriseus ) is a 16-membered macrocyclic lactones antibiotics, which belongs to milbemycin subfamily. Moxidectin is chemically synthesized from nemadectin, which has broad application prospects on agricultural insecticides field because of its high efficiency, safety, environmentally friendly and so on. Therefore, many researches have paid more attention in the past few decades and most of the researches were focused on genomic, chemical modification and pharmacological aspects, such as the global genome sequence ananlysis of
S. cyaneogriseus, and toxicology of moxidectin on the animal deworming. However, any researches hardly focused on the fermentation technology of process optimization and regulation of nemadectin fermentation are scarce up to now. In this study, soybean oil had a positive effect nemadetin fermentation. The biomass of
S. cyaneogriseus was effectively improved by adding soybean oil at start in 5 L fermentation (for about 29%), and the titer of nemadectin rised up to 1 475 μg/mL, which was increased by 27.37%. Based on the analysis of the fermentation parameters, The primary metabolic activity of
S. cyaneogriseus and the synthesis rate of nemadectin was greatly improved by the addition of soybean oil at the start of fermentation, resulting in a significant increase in the total yield of nemadectin.