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    程雅文, 豆伟涛, 陈国荣. 二硫化钼复合材料的构建与对Aβ的灵敏检测[J]. 华东理工大学学报(自然科学版), 2019, 45(5): 759-766. DOI: 10.14135/j.cnki.1006-3080.20180720001
    引用本文: 程雅文, 豆伟涛, 陈国荣. 二硫化钼复合材料的构建与对Aβ的灵敏检测[J]. 华东理工大学学报(自然科学版), 2019, 45(5): 759-766. DOI: 10.14135/j.cnki.1006-3080.20180720001
    CHENG Yawen, DOU Weitao, CHEN Guorong. Development of MoS2 Composites and Sensitive Detection of Amyloid β-Protein[J]. Journal of East China University of Science and Technology, 2019, 45(5): 759-766. DOI: 10.14135/j.cnki.1006-3080.20180720001
    Citation: CHENG Yawen, DOU Weitao, CHEN Guorong. Development of MoS2 Composites and Sensitive Detection of Amyloid β-Protein[J]. Journal of East China University of Science and Technology, 2019, 45(5): 759-766. DOI: 10.14135/j.cnki.1006-3080.20180720001

    二硫化钼复合材料的构建与对Aβ的灵敏检测

    Development of MoS2 Composites and Sensitive Detection of Amyloid β-Protein

    • 摘要: 为解决传统检测阿兹海默症标志物Aβ的荧光方法普遍存在灵敏度低、背景干扰大以及操作过程繁琐的缺点,通过超分子自组装的方法将3种不同波长的荧光探针(白藜芦醇(Res)、硫磺素T(ThT)和4-(二氰基亚甲基)-2-甲基-4H-吡喃衍生物(DCM-OH))与二硫化钼进行复合,通过高分辨质谱和高效液相色谱表征了3种化合物的结构以及纯度,利用扫描电镜和透射电镜研究了复合材料的形貌结构,并对两种不同分子量的肽段片段Aβ(1~40)和Aβ(1~42)进行了检测。结果显示:复合材料结构规整均一,3种体系均能实现对Aβ(1~40)和Aβ(1~42)的灵敏检测;通过合成得到的DCM-OH与MoS2的复合体系粒径分布均一,生物相容度好,对Aβ(1~42)有更低的检测下限(6.8 nmol/L),具有潜在应用价值。

       

      Abstract: Amyloid beta peptide (Aβ) has been considered as the hallmark of Alzheimer’s disease which is neurodegenerative disease and affects many senior citizens. However, drug for the treatment or control of such a disease is still lacking, thus early detection and intervention are particularly important. So far, fluorescence diagnose technique has been developed as an important mean for the detection of Aβ, but there are still many disadvantages in the traditional fluorescence techniques, such as low sensitivity, large background interference, and complicated operation. To address these issues, we synthesized a series of dicyanomethylene- 4H-pyran (DCM-OH) derivatives which were self-assembled with molybdenum disulfide (MoS2) driven by π-π stacking interaction. The commercial products, resveratrol (Res) and thioflavine T (ThT), were used as the control group. The structures and purities of the three compounds were characterized by high resolution mass spectrometry (HRMS) and high performance liquid chromatography (HPLC). The morphologies and structures of the three composites were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The characterization results display that the three prepared composites are regular and uniform, and can be used to detect indicators of AD in vitro. Moreover, we tested the applicability of the three composites in the detection of Aβ (1−40) and Aβ (1−42) in solution. The results show that the composites are capable of detecting Aβ (1−40) and Aβ (1−42) sensitively. In addition, the composite DCM-OH/MoS2 was more sensitive for the detection of Aβ (1−42) with a LOD (Limit of Detection) of 6.8 nmol/L, which is 9.8 nmol/L for Aβ (1−42). Finally, we also tested the selectivity of the composite DCM-OH/MoS2 against other proteins, and found that it showed better selectivity for Aβ. The results demonstrate that the developed system shows certain biological application prospects.

       

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