Abstract: Curdlan has been widely applied in food, medicine, and aquaculture due to its unique gelation and immune-enhancing properties. Curdlan is mainly produced by microorganism fermentation in order to meet the market demand. The curdlan fermentation broth is a kind of highly viscous fluid, and the viscosity increases significantly with the curdlan accumulation during fermentation process, resulting in poor mixing and mass transfer efficiency and subsequently limiting the biosynthesis of curdlan. This study investigated the effects of glucose control modes (batch, pulse fed-batch, continuous fed-batch) and impeller combination types (triple Rushton turbines (3RT), bottom Rushton turbines combined with upper Ellipse (RT+E)) on the metabolic characteristics of curdlan fermentation using glucose as a carbon source for the biosynthesis of curdlan. The results showed that the curdlan biosynthesis of Alcaligenes faecalis was significantly inhibited at the initial higher glucose concentration in batch fermentation, and the yield of curdlan was 25.00 g/L. The inhibition effect on the curdlan biosynthesis of Alcaligenes faecalis led by high glucose concentration could be remarkably reduced by using a continuous fed-batch strategy with a concentration of the glucose at around 20 g/L, and the yield of curdlan was enhanced to 33.88 g/L. In addition, the RT+E combination was demonstrated to be an effective approach for reducing the mixing time of the fermentation broth. Finally, the yield of curdlan could be further increased to 36.10 g/L, leading to a 44.40 % increase compared to that obtained in batch fermentation.