Abstract:
A method for separation of (6,5)chiral single-wall carbon nanotube (SWCNT) was reported. The separation was achieved by an aqueous two-phase system (ATP) consisting of polyethylene glycol/dextran in the presence of hydrochloric acid (HCl) and surfactants. Paclitaxel (PTX) has been used in the clinical treatment of breast cancer, ovarian cancer, and etc., which has poor water solubility and low toxic side effects. The non-covalent adsorption of PTX with bovine serum albumin (BSA) resolves the issue of poor water solubility for PTX. The PTX adsorbed BSA can also be coated on the surface of SWCNTs to become a (6,5)SWCNT-BSA-PTX ternary complex through non-covalent modification of SWCNTs, which leads to the enhancement of cell penetration. The cytotoxicity of the ternary complex was evaluated and the results showed that (6,5)SWCNT as a promising drug carrier could significantly reduce the IC
50 of PTX to 7.1×10
-9 mol/L. It can be concluded that the method established in this work does not require complex covalent functionalization of SWCNTs and biocompatibility of the materials is significantly improved by involving BSA. The toxic effects of PTX is greatly improved for HeLa cells by separated single chiral (6,5)SWCNTs, which should have applications for delivery of other drugs.