Abstract: By analyzing the properties of probe measurement system for single molecule detection of nanopore in detail, mainly including the structure of the probe and the measurement bandwidth, this paper designs an improved high-precision acquisition probe system by utilizing the pre-compensation method. The objective of the acquisition probe system is the efficient acquisition of the original signal in nanopore single-molecule detection system, in which the input signal is pA-level current. In order to measure small current signals, it is necessary to use a high-gain operational amplifier as widely used in traditional nanopore probe system. However, there are two main shortcomings in traditional nanopore probe system, i.e., the insufficiency of the system measurement bandwidth (only a few kHz) and too much noise in the current signal. These are mainly due to the value of the feedback resistance in the operational amplifier circuit to be too huge (usually reaching a few GΩ). The parasitic capacitance of the large resistor will affect the bandwidth of system and the thermal noise of the large resistor will increase the circuit noise of the system. Especially, the resistance value has positive correlation with the noise of the circuit and negative correlation with the bandwidth of the system. Hence, it is quite important to select an appropriate resistance value when we consider these factors. The value of the feedback resistance in the designed probe system is 200 MΩ. The designed acquisition probe system can not only expand the measurement bandwidth of the original measurement system, but also flexibly configure zero point and pole point of the system to speed up the dynamic response process of the system. Finally, the feasibility and effectiveness of the proposed method are verified by simulation experiments through the actual probe circuit.