Abstract:
Marine environment with high pressure, high salt, hypoxia and low light intensity is quite different from that of the land, which allows that marine microorganisms often have special physiology, growth and production behaviors.
Aspergillus glaucus HB1-19 is a typical marine-derived fungus that can tolerate high salt stress. The developmental pattern of filamentous fungi usually switches between an asexual and sexual mode, correlating with secondary metabolism and switching in response to numerous environmental signals including light, ambient pH, nutrient availability, temperature, and osmolality. Our previous results showed that salt stress inhibited its sexual development but promoted asexual one at the same time. Filamentous fungi easily response to light signal and regulate cell development. A global regulation factor VeA and its protein complex related proteins of FphA, LreA and LreB usually function in this physiological process. This work aims to investigate whether or not the VeA protein responses to salt stress signal and regulates the cell development transitions. Genome walking is firstly used to clone the flanking gene sequences of
AgveA, AgfphA, AglreA and
AglreB, and then introns of these genes are confirmed by reverse transcription. The results of qRT-PCR show that the transcription of
AgveA is obviously up-regulated by salt stress, while
AgfphA, AglreA and
AglreB are down-regulated. Afterwards,
AgveA is knocked out and a gene deficient strain of Δ
AgveA is constructed to further study the functions of
AgveA in
A. glaucus. The morphology of mycelial morpholo and colony, mycelial growth rate and cell development phonotypes are evaluated. The mycelial morphology is little changed while some edge defects are found in colonies. Mycelial growth rate accelerates in the late stages of development. Deletion of
AgveA results in the blockage in production of cleistothecia under high salt stress, while it produces many conidiosprores and simultaneously a spot of cleistothecia under low salt stress.