Abstract:
An open tubular capillary liquid chromatography column (OTCLC) coated by polyglycidyl methacrylate-
co-poly(ethylene glycol) diacrylate (GMA-
co-PEGDA) was prepared by surface initiated atom transfer radical polymerization (SI-ATRP).The polyethylene glycol grafting resulted in the formation of bulk structure,obviously increasing the phase ratio and column capacity.Characterized by scanning electron microscopy (SEM),the monolithic layer was observed to be rough.Four small molecules including 4-hydroxy benzoic acid,benzoic acid,2,4-dihydroxy benzoic acid and benzene were used to evaluate the separation performance of the open-tubular capillary liquid chromatography column.The results showed that bulk structure of the poly(GMA-
co-PEGDA) OTCC not only remained the characteristic of fast separation but also provided more active sites.The run-to-run (
n=5) relative standard deviations (RSDs) for retention time for 5 consecutive injections of 4 molecules were less than 2.5%,indicating good repeatability.Finally,the OTCC was applied to the separation of protein mixtures,and also obtained good separation ability.